藥毒所96年度

2007 Annual Report vip3Aa 2.4 kb vip3Aa amplified from B11-30a by PCR vipF30 vipR33 pSC-A 3.5 kb lac Z' bla lac promoter pUC ori f1 ori PCR Product Insertion site loxP T5 promoter bla pQE82 4.7 kb Hind II Pst I Sal I Sma I Kpn I Sac I Sph I Bam HI Ligation pQE-vip3Aa 7.1 kb T5 promoter vip3Aa (2.4kb) bla pSC-A-vip3Aa 5.9 kb vip3Aa bla lac promoter pUC ori f1 ori loxP Bam HI Sph I 圖 26. pQE- vip3Aa 之質體構築。