臺灣農藥科學第四期電子書

臺灣長豇豆花器葉化菌質體 67 Identification and Characterization of Phyllody Phytoplasma Associated with Vigna sesquipedalis in Taiwan Che-Ming Chang 1 , Fuh-Jyh Jan 2 , Chiou-Chu Su 1* Abstract Chang, C. M., Jan, F. J., and Su, C. C. 2018. Identification and characterization of phyllody phytoplasma associated with Vigna sesquipedalis in Taiwan. Taiwan Pestic. Sci. 4:53-67. In July 2015, Vigna sesquipedalis (cowpea plants) with phytoplasma-like symptoms were found in Shuilin Township, Yunlin County in Taiwan. The systemic symptoms on these plants included phyllody, witches’-broom, the proliferation of small leaves, and leaf yellowing. Transmission electron microscopy, PCR detection, 16S rDNA sequence analysis, and virtual restriction fragment length polymorphism (RFLP) were used to identify the pathogen, which was determined to be phytoplasma. Micrographs revealed pleomorphic phytoplasma particles in the sieve tubes of symptom V. sesquipedalisa . Nested PCR assays with P1/P7 and R16F2n/R16R2 universal phytoplasma primers further detected a 1.8 kb product and an 1.2 kb product. A phylogenetic tree using the maximum likelihood method and virtual RFLP analysis with an i PhyClassifier based on 16S rDNA both revealed that this cowpea phyllody phytoplasma belongs to 16SrII-A group . Key words: Vigna unguiculata , phytoplasma, phyllody. Accepted: June 22, 2018. * Corresponding author, E-mail: auba@tactri.gov.tw 1 Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Taichung 2 Department of Plant Pathology, National Chung Hsing University, Taichung