農藥所111年度

表 12. 本研究進行 TaqMan PCR 偵測 Xt 之梨樹葉片 DNA 來源及病徵狀態 a Ct ≤ 30 was selected as a threshold for an Xt positive call 表 13. 5 組 TaqMan PCR 引子偵測 10 個 PLS 葉片 DNA 之 Ct 值 TaqMan qPCR Xt803-F/R Xt731-F/R Xt16S-F/R XtgB1-F/R XtgB2-F/R Total DNA Ct Xt DNA Ct Xt DNA Ct Xt DNA Ct Xt DNA Ct Xt DNA XtgB1-R Reverse GGTCGTCGTT AGTGTAGGAC XtgB1-F Forward CGATGCTGAT GTGGATGGTT 111 DNA gyrase gene ( gyrB ) 1 6564-6674 XtgB1-P Probe CGGCAAATGC CAGAACTGAT TGAACGT XtgB2-R Reverse AAGGGTGGTA AGCCAATGTA G County Orchard Sample number Scorch symptom Xt isolation Standard PCR (PLS-F/R) TaqMan qPCR (Xt731-F/R) a Yes No + Tries + - + - Not done Yilan A 4 4 0 0 0 1 3 4 0 0 B 10 8 2 0 8 4 6 6 2 2 Hsinchu C 2 2 0 0 2 0 2 0 0 2 D 10 10 0 0 10 4 6 8 0 2 Miaoli E 14 10 4 6 10 7 7 8 4 2 F 16 16 0 6 6 10 6 10 0 6 G 4 4 0 0 0 0 4 0 0 4 H 14 14 0 4 6 11 3 12 0 2 I 8 8 0 6 6 7 1 8 0 0 J 6 6 0 6 6 6 0 6 0 0 K 4 4 0 2 2 3 1 4 0 0 Taichung L 4 0 4 0 0 0 4 0 1 3 M 2 2 0 0 0 2 0 2 0 0 N 2 2 0 0 0 1 1 2 0 0 O 2 2 0 0 2 0 2 0 0 2 P 2 2 0 0 2 0 2 0 0 2 Q 6 6 0 5 6 5 1 4 0 2 R 6 6 0 0 0 4 2 4 0 2 S 4 4 0 0 4 3 1 4 0 0 T 4 4 0 2 2 2 0 4 0 0 U 4 4 0 2 2 2 2 4 0 0 V 2 2 0 2 2 0 2 2 0 0 W 10 10 0 4 4 7 3 10 0 0 Total 23 140 130 10 44 80 79 61 102 7 31 53