137 / 220
茼蒿細菌性葉斑病
135
(GenBank accession number: KU884652-
KU884654)
， 並 將 所 得 序 列 於
BLAST
網
站進行序列比對，結果顯示供試菌株皆與
P. syringae
pv.
antirrhini
(GenBank accession
number AY342166)
之序列相同度達
99%
。
各菌株之
16S-23S ITS
的序列片段為
418 ~
429 bp
，長度變化為
12 bp
，進一步對照序
列比對結果，分離自茼蒿的本病原菌與
P.
syringae
pv.
antirrhini
、
P. syringae
pv.
toma-
to
、
P. syringae
pv.
maculicola
及
P. syringae
pv.
lachrymans
之
16S-23S ITS
核苷酸序列相似
度可達
99.5 ~ 100%
。將此序列以鄰聚連結
表三、茼蒿細菌性葉斑病菌之生理生化特性
Table 3.
Physiological and biochemical characteristics of
Psedomonas syringae
strains obtained
from
Chrysanthemum coronarium
Characteristic
Tested strain
P. syriangae
1)
P. cichorii
1)
KOH test
G(-)
G(-)
G(-)
Fluorescent pigment on KB
+
2)
+
+
Flagellar number
≥ 1
≥ 1
≥ 1
Tobacco HR
+
+
+
Growth at 41
°
C
–
–
–
Potato rot
–
–
–
Levan
+
+
–
Arginine dihydrolase
–
–
–
Utilization of:
Manitol
+
+
+
Geraniol
–
–
–
Benzoate
–
–
–
Cellobiose
–
–
–
Sorbitol
+
+
–
Trehalose
–
–
–
Sucrose
+
+
–
m-Tartrate
+
V
+
D-Tartrate
+
V
–
D-Arabinose
–
–
–
L-Rhamnose
–
–
–
1)
Identified according to laboratory guides for the identification of plant pathogenic bacteria by Braun-Kiewnick
and Sands (2001)
(7)
and Schaad (1988)
(27)
.
2)
+: positive reaction; –: negative reaction.