133 / 220
茼蒿細菌性葉斑病
131
eter
模式，計算兩兩序列間之遺傳距離，並
以鄰近連結法
(neighbor-joining method)
(24)
方
法繪製親緣關係樹圖，以
1,000
次重複取樣
之
bootstrap replications
值以檢驗親緣關係樹
狀圖各支點的可信度
(10, 17)
，比較各不同的病
原型之親緣關係結果。
十、藥劑感受性測定
以濾紙擴散法於
NA
平板培養基上測定
供試細菌對之感受性，選取前述
6
個菌株作
為供試菌株，菌株濃度調整為
10
8
cfu/mL
之
菌液。以噴霧方式將菌液均勻噴灑於
NA
平
板培養基上，將供試藥劑稀釋成不同濃度
表一、
16S-23S ITS
序列比對所使用的茼蒿細菌性葉斑病菌及其他
Pseudomonas syringae
病
原型菌株
Table 1.
The 16S-23S ITS sequence alignment for strains of
Pseudomonas syringae
and other
pathovar types obtained from
Chrysanthemum coronarium
Strain
Number
GenBank accession number
Genomospecies
1)
Pseudomonas syringae
Cc39
KU884652
G3
Cc24
KU884653
G3
Cc30
KU884654
G3
pv.
antirrhini
ICMP 4303
AY342166
G3
pv.
aptata
ICMP 4387
AY342167
G1
pv.
pisi
PP105
AY850197
G1
pv.
tabaci
ICMP 2835
AY342183
G2
pv.
tagetis
ICMP 4091
AY342202
G7
pv.
helianthi
ICMP 4531
AY342173
G7
pv.
syringae
NRRL B-1631
AY342181
G1
pv.
coronafaciens
ICMP 3113
AY342169
G4
pv.
porri
BD 450
AY644714
G4
pv.
garcae
ICMP 4323
AY342171
G4
pv.
delphinii
ICMP 529
AY342170
G3
pv.
lachrymans
LMG 5070
JN624860
G3
pv.
actinidiae
ICMP 9617
AY342165
G3
pv.
maculicola
10
AY342174
G3
pv.
morsprunorum
LMG5075
EU009174
G3
pv.
passiflorae
NZ187
GU808449
G3
pv.
tomato
DC3000
AY342209
G3
1)
Genomespecies grouping was referenced from Gardan
et al.
(1999)
(11)
.