臺灣農藥科學第一期

臺灣農藥科學　第

期

thymidine (T) at position 532, whereas the susceptible biotype had a cytosine (C) at that

position. Moreover, the amino acid at position 178 is the major target site among glyphosate

resistant grasses around the world, and transcribed sequence data revealed that amino

acids at this position were serine178 and proline178 for resistant and susceptible biotypes,

respectively. Finally, we designed two sets of primers with reference to 532 bases and

Hae

III

specific to EPSPS nucleotides of resistant and susceptible

H. corymbosa.

These primers were

used in single nucleotide polymorphism (SNP) and Polymerase Chain Reaction-Restriction

Fragment Length Polymorphism (PCR-RFLP) assays for detecting of

H. corymbosa

collected

from southern Taiwan. We found that 4 out of 11

H. corymbosa

populations possessed

resistant biotypes and were clearly distinguishable from the 7 populations with susceptible

biotypes.

Key words:

glyphosate, resistance,

Hedyotis corymbosa

, EPSP synthase activity, point

mutation, molecular identification.