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增益植物健康之多功能蘇力菌研究
69
Characterization Assay of Multifunctional
Bacillus
thuringiensis
Strains with the Ability to Enhance
Plant Health
Fei-Ting Hu
1
, Sheueh Kuo
1
, Mi-Hau Tsai
1
, Ching-Chou Tzeng
1
*
Abstract
Hu, F. T., Kuo, S., Tsai, M. H., and Tzeng, C. C. 2016. Characterization assay of multifunctional
Bacillus
thuringiensis
strains with the ability to enhance plant health. Taiwan Pestic. Sci. 1: 50-69.
In this study we investigated several properties of
Bacillus thuringiensis
(
Bt
) strains
with good pest killing effects, such as plant growth promotion, pathogen suppression,
etc. After performing PCR on five strains of
Bt
(subsp.
aizawai
[
Bta
] A603,
Bta
Ab12,
Bta
ABTS1857, and
Bt
subsp.
kurstaki
[
Btk
] E911, and
Btk
ABTS351), we detected genes for
phosphate solubilization, indole-3-acetic acid (IAA), siderophore, 1-aminocyclopropane-
1-carboxylate deaminase (ACCD), chitinase, and
β
-1,3-glucanase. Physiological analyses
showed that (1) all strains could produce IAA, phosphorus solubilization, urease, chitinase,
and nitrate reductase, and (2) IAA and urease in high salinity were secreted. The results of
pot experiments further indicated that
Bt
could enhance the plant growth, improve root
development, increase the efficiency of fertilizer, promote salinity tolerance, and reduce
pathogen invasions. Finally, we found that
Bt
was efficient at increasing the fresh weight of
cabbage in the field. In summary, this study confirmed that
Bt
can confer multiple benefits
to agriculture, such as, suppressing plant pathogens, alleviating soil salinity stress, and
improving the health, quality, fertility, and productivity of crops.
Key words:
Bacillus thuringiensis
, plant growth promotion, microorganism inhibition,
multifunctional microorganism.
Accepted: September 12, 2016.
* Corresponding author, Email:
cctzeng@tactri.gov.tw1
Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture. Taichung.