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臺灣農藥科學 第
1
期
190
餅及棉籽粕樣品中，皆檢出棉酚殘留，除
4
件棉籽樣品殘留量為
6,347 ~ 7,325 ppm
，其
餘檢測結果符合歐盟對棉籽、棉籽殼及棉
籽餅中的棉酚含量規定棉籽
≤ 5,000 mg/kg
、
棉籽殼及棉籽餅
≤ 1,200 mg/kg
；在
12
件
泌乳牛料、肉鴨飼料及肉雞飼料樣品中，
於
2
件泌乳牛料中檢出棉酚殘留，其檢測
結果符合歐盟對於牛每日飼料中的棉酚含
量限制值分別為：成年牛
≤ 500 mg/kg
、犢
牛
≤ 100 mg/kg
，其他不含棉籽粕的飼料需
表一、
5
種分析方法之儀器最低偵測極限與定量極限之比較
Table 1.
Comparison of IDL and LOQ for five methods studied
Method
Method I
Method II
Method III Method IV Method V
Instrument
Spectrophotometric
determination
HPLC LC/MS/MS LC/MS/MS LC/MS/MS
Sample
cottonseed, cotton-
seed hulls, and cot-
tonseed meal
feedstuffs mushrooms forest mush-
room com-
post
milk
Instrument detection
limit (mg/L)
0.8
0.05
0.001
0.002
0.005
Limit of quantita-
tion (mg/kg)
100
5
0.05
0.02
0.01
表二、不同基質與方法中棉酚回收率測試結果
Table 2.
Gossypol recovery results from different methods and matrixes
Type of sample
Method
Low concentration
High concentration
Spike level
(mg/kg)
Recovery
(%)
CV
(%)
Spike level
(mg/kg)
Recovery
(%)
CV
(%)
Cottonseed
I
2,500
87
12
5,000
82
6
Cottonseed hulls
I
500
100
4
1,000
99
6
Cottonseed meal
I
500
100
2
1,000
98
8
Feedstuffs for adult
cattle
II
100
95
1
200
92
3
Feedstuffs for poultry
(chicken)
II
100
93
2
200
94
1
Mushrooms
III
0.25
67
8
1
77
1
Forest mushroom
compost
IV
0.2
81
4
1
84
3
Milk
V
0.1
81
18
1
73
7